Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Electron Microscopy Sciences USAF Test Chart R75P Positive Image
USAF resolution charts are recognized the world over as a universal standard for testing the vertical and horizontal resolution of imaging systems. Each element on the chart comprises three vertical bars and three horizontal bars, and the detail on these slides is as fine as 0.78microns (644 line-pairs per mm). The resolution of the imaging system is normally specified as the Group and Element of the finest bars that can be clearly defined. Sub Micron smallest feature size - bar width 0.00078mm [0.78 um].
Type: Positive Image
Use: Groups 0/1 to 9/3
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES LX-2 CELL LINE HUMAN HEPATIC
NC3816670 LX-2 CELL LINE HUMAN HEPATIC
Electron Microscopy Sciences Nagoette Single Counting Cell 0.5 mm
Counting cells are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting. All complete with case and 1 pair of cover glasses. The depth of the Nageotte counting is 0.5 mm. For Liquids in Poor content.
Electron Microscopy Sciences Pipette Tip Universal, 5 - 200 mcL
Pipette tips are low binding to ensure accuracy and precision. The low binding surface is the result of a manufacturing process that binds a perfluorooctyl polymer to polypropylene resin–resulting in an exposed trifluoromethyl surface. This surface reduces the surface energy of liquid and repels any substance so sample retention and binding are minimized.
Not detectable/Non-reactive.
Extracted only by a fluorinated solvent.
Autoclaving will not compromise performance.
Available in convenient hinged racked package.
Tips and racks are fully autoclavable. Natural tips feature reference marks at 10 ul, 50 ul, and 100 u. Low binding surface.
Range: 5 - 200 µl
Molecular Devices Absorbance Filter, 450 nm, 8 nm, FilterMax Compatible
Replaceable narrow-band absorbance (photometric) filter designed for use in FilterMax multimode microplate readers. The filter is centered at 450 nm and has an approximate 8 nm bandwidth, providing a specific narrow bandpass for fixed-wavelength absorbance measurements in microplates.
- Center wavelength: 450 nm.
- Bandwidth: 8 nm (approximate).
- Designed for fixed-wavelength absorbance assays.
- Installs in the instrument filter wheel for modular wavelength selection.
- Narrow-bandpass optical filter for consistent photometric readings.