Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Ace Glass, Inc. Adapter, 0-10 Hi-Vac valve, no.15 O-ring joint to #15Ace-Thred, No-Air
ADAPTER VAC 0-10VLV NO15-NO15
Dojindo Molecular Technologies Inc ATP Assay Kit-Luminescence (50 tests) is used to determine intracellular levels of ATP by luciferase luminescence assay. High-sensitivity detection can be performed with stable luminescence. 50 tests
Adenosine triphosphate (ATP) is an energy source for living cells and is synthesized by both glycolysis and mitochondrial oxidative phosphorylation. Decreased ATP levels are associated with cancer, aging, neurodegenerative diseases, and mitochondrial diseases. The ATP Assay Kit-Luminescence enables the quantitation of intracellular ATP by luciferase luminescence assay. This kit can be applied for microplate assay. Without a complicated process including medium removal and cell washing, the ATP Assay Kit-Luminescence can generate a stable luminescent signal with a half-life of greater than 3 hours by simply mixing the kit components and adding the reagent. This kit contains an ATP Standard, which provides highly reproducible data without measuring unstable ATP. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Molecular Devices Emission Filter, Time Resolved, 490 nm, 10 nm
Time-resolved emission filter designed for time-resolved fluorescence measurements. The optical filter centers at 490 nm with a 10 nm full width bandwidth and is supplied with a holder for insertion into compatible multi-mode microplate readers. Typical applications include donor emission detection in TR-FRET and other time-resolved assays.
- 490 nm center wavelength, 10 nm bandwidth.
- Includes holder for filter-slide insertion.
- Designed for time-resolved fluorescence applications.
- Optimized for donor emission detection in TR-FRET assays.
- Fits standard filter slots on compatible microplate readers.
Molecular Devices SpectraDrop Micro-Volume Refills, 24-Well, Teflon-Coated, Pack of 3
Molecular Devices SpectraDrop Micro-Volume 24-well refill kit includes three low-volume, Teflon-coated bottom slides to replace worn slides in SpectraDrop micro-volume assemblies. Slides are designed for precise droplet placement and reproducible low-volume absorbance and quantitation using 2 μL or 4 μL configurations.
- Contains three low-volume 24-well bottom slides.
- Teflon-coated bottom slides for precise droplet placement.
- Compatible with micro-volume adapters and cover slides.
- Supports 2 μL and 4 μL low-volume configurations (system-level).
- Intended for micro-volume absorbance and sample quantitation.
Molecular Devices Nikon Plan Apochromat Lambda D Objective, 2x, 0.10 NA, Air
The CFI Plan Apochromat Lambda D 2x objective is a high-performance, infinity-corrected microscope objective designed for wide-field life-science imaging. It delivers a flat, low-aberration image across a large 25 mm field for brightfield and fluorescence workflows.
- Magnification: 2x.
- Numerical aperture: 0.10.
- Working distance: 8.5 mm.
- Immersion type: air.
- Objective type: plan apochromat.
- Cover glass thickness: 0-0.17 mm.
- Applications: brightfield, DIC, fluorescence, confocal, TIRF.
Molecular Devices Fluorescence Filter Kit, EM 660 nm, 40 nm
The SpectraMax Fluorescence Intensity Filter Kit delivers five exchangeable optical filters for fluorescence, luminescence, and time-resolved fluorescence (TRF) assays on compatible multi-mode microplate readers. The kit includes an emission 660 nm filter with a 40 nm bandwidth plus additional excitation and emission filters to support common assays.
- Includes five exchangeable filters covering common fluorescence wavelengths from ~400-700 nm.
- Contains an emission 660 nm filter with a 40 nm full bandwidth for near-IR fluorescence detection.
- Supports fluorescence intensity, luminescence, and time-resolved fluorescence assay modes.
- Allows quick filter exchange to adapt to different assay requirements.
- Designed for use with compatible multi-mode microplate readers to expand detection flexibility.
Molecular Devices Fluorescence Polarization Filter, 485 nm, 20 nm, Vertical
The Molecular Devices fluorescence polarization filter (MPN 6590-0094) is a vertical polarization filter engineered for fluorescence polarization assays with a 485 nm center wavelength and a 20 nm optical bandwidth. It is intended as a replacement or accessory filter for compatible multi-mode microplate readers.
- Optimized for fluorescence polarization assays at a 485 nm center wavelength.
- 20 nm bandwidth provides narrow spectral selection and reduced crosstalk.
- Vertical polarization orientation for polarization-based measurements.
- Designed for installation in filter-slide assemblies of compatible microplate readers.
- Replacement filter for restoring or modifying detection channels.
Molecular Devices Fluorescence Excitation Filter, 550 nm, 10 nm
A narrow-band fluorescence excitation filter centered at 550 nm with a 10 nm full width at half maximum (FWHM), designed for microplate fluorescence assays to isolate excitation light and improve signal specificity.
- Provides narrow-band excitation centered at 550 nm.
- 10 nm full width at half maximum for selective excitation.
- Optimized for microplate fluorescence measurements.
- Reduces background and improves signal-to-noise ratio.
- Designed for use in compatible microplate fluorescence readers.
Molecular Devices RS-232C Cable, DB-25 Male to DB-9 Female, Straight-Through
This dedicated RS-232C serial cable connects MAXline series microplate readers (EMax, VMax, UVMax, ThermoMax) to a computer RS-232 port. It uses a DB-25 male connector at the instrument end and a DB-9 female connector at the computer end and follows the manufacturer's straight-through RS-232C pin routing for reliable bidirectional communication.
- Connector type: DB-25 male (instrument) to DB-9 female (computer).
- Interface: RS-232C, straight-through wiring.
- Shielding: double-shielded cable recommended for noise reduction.
- Compatibility: MAXline family microplate readers with DB-25 RS-232 instrument port.
- Pin wiring: manufacturer-specified 25-to-9 pin routing for bidirectional communication.
- Cable length: not specified in available documentation.